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Catalog#: BX51
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Generation of Fusarium graminearum Knockout Mutants by the Split-marker Recombination Approach
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Date:
2018-08-20
[Abstract]  Fusarium graminearum is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by applying “split-marker” deletion strategy (Catlett et al., 2003) with PEG-mediated protoplast transformation (Yuan et al., 2008; Martín, 2015). [摘要]  禾谷镰刀菌是一种破坏性的植物病原体,具有令人印象深刻的代谢多样性。 基因缺失是基因功能研究的重要且有用的方法。 在这里,我们提出了一个协议,通过应用“分裂标记”删除策略(Catlett et al。,2003)与PEG介导的原生质体转化(Yuan 等。,2008;Martín,2015)。

Perithecium Formation and Ascospore Discharge in Fusarium graminearum
Author:
Date:
2018-08-05
[Abstract]  The filamentous ascomycete Fusarium graminearum (previously also known as Gibberella zeae) is a phytopathogen of grain cereals, reducing crop yield and grain quality. The abilities of sexual reproduction organ-perithecium formation, ascospore formation and discharge are all essential characteristics relevant to F. graminearum disease cycle. Here, we present the details of the protocol to study perithecium formation and ascospore discharge in F. graminearum. [摘要]  丝状子囊菌 Fusarium graminearum (以前也称为 Gibberella zeae )是谷物谷物的植物病原体,降低了作物产量和谷物质量。 有性生殖器官 - 外膜形成,子囊孢子形成和排出的能力都是与 F相关的基本特征。 禾本科疾病周期。 在这里,我们提出了研究 F中的perithecium形成和子囊孢子放电的协议的细节。菌。

【背景】子囊菌真菌 Fusarium graminearum 是小麦 Fusarium 头枯病和玉米赤霉病茎腐病的主要致病因子。这种真菌可以在定植的寄主植物表面产生有性子实体 - perithecia,在农作物碎片上过冬,并在明年流行时排出子囊孢子(Goswami和Kistler,2004)。受潮湿和温暖条件的影响,子囊孢子被强制从子囊中排出,并在气流中作为主要接种物空气传播。

这种真菌是同种异体的;大多数菌株可以很容易地在体外上生产胡萝卜琼脂上的perithecia(Trail and Common,2000)。已经报道了在 F的性发育期间的时间转录组学分析的微观研究和对perithecia发展的全面描述。 graminearum (Trail and Common,2000; Hallen et ...

Fusarium graminearum Double (Triple) Mutants Generation Using Sexual Crosses
Author:
Date:
2018-08-05
[Abstract]  Fusarium graminearum is a destructive phytopathogen that infects major cereal crops such as wheat, maize and barley. Double or triple mutants are often very useful in the phenotypic and genetic analysis of genes that function redundantly or within similar pathways. When single gene mutants are available, double or triple mutants can be generated by crossing heterothallic strains or multiple rounds of protoplast transformation. When individual mutants carry different antibiotic resistance, it is convenient to use the sexual crossing to generate desired recombinant strains. Here, we present a protocol for generating double or triple mutants by sexual crossing in one homothallic strain with further antibiotic resistance and genomic DNA PCR screening of recombinant progenies. [摘要]  禾谷镰刀菌(Fusarium graminearum)是一种破坏性的植物病原体,可感染小麦,玉米和大麦等主要谷类作物。 双重或三重突变体通常在冗余或相似途径内起作用的基因的表型和遗传分析中非常有用。 当可获得单基因突变体时,可通过杂交异源菌株或多轮原生质体转化产生双突变体或三突变体。 当单个突变体携带不同的抗生素抗性时,使用有性杂交方便产生所需的重组菌株是方便的。 在这里,我们提出了一种方案,通过在一个同源的菌株中通过有性杂交产生双突变体或三突变体,具有进一步的抗生素抗性和重组后代的基因组DNA PCR筛选。

【背景】子囊菌真菌镰刀菌(Fusarium graminearum)是一种破坏性的植物病原体,可引起谷类食物的头部枯萎病,穗腐病,茎腐病和冠。它可以在胡萝卜琼脂体外产生perithecia 。该测定法可用于研究子囊膜发育,子囊孢子放电和性重组(Nicholson,2007)。

F。禾本科植物是同源的,具有MAT1-1和MAT1-2-1基因座;这些基因座缺失突变体中的每一个在自交中都是无菌的(Zheng et al。,2013)。为了产生双基因突变体,一个单基因突变体传统上与 MAT 缺失突变体杂交,并进一步与另一个突变体杂交(Bowden和Leslie,1999; Lee et al。 ,2011; Son et ...

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