Hydroxyurea
Company: Sigma-Aldrich
Catalog#: H8627
Product evaluation:
Single-molecule Analysis of DNA Replication Dynamics in Budding Yeast and Human Cells by DNA Combing
Author:
Date:
2017-06-05
[Abstract]  The DNA combing method allows the analysis of DNA replication at the level of individual DNA molecules stretched along silane-coated glass coverslips. Before DNA extraction, ongoing DNA synthesis is labeled with halogenated analogues of thymidine. Replication tracks are visualized by immunofluorescence using specific antibodies. Unlike biochemical and NGS-based methods, DNA combing provides unique information on cell-to-cell variations in DNA replication profiles, including initiation and ...

DNA Damage Sensitivity Assays in Caenorhabditis elegans
Author:
Date:
2015-06-05
[Abstract]  C. elegans has served as a genetically tractable multicellular model system to examine DNA damage-induced genotoxic stress which threatens genome integrity. Importantly, the high degree of conservation shared between worms and humans offers the advantage that findings about DNA damage-induced cell cycle arrest/checkpoint response and DNA double-strand break repair in worms are applicable to human studies. Here, we describe simple DNA damage sensitivity assays to quantify the response ...

DNA Damage Sensitivity Assays with Arabidopsis Seedlings
Author:
Date:
2014-04-05
[Abstract]  We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a radiomimetic drug, (3) mitomycin C, a DNA intrastrand cross-linker, (4) hydroxyurea, an inhibitor of DNA synthesis and (5) UV-C, which causes mainly photoproducts. The “true leaf assay” and the “UV resistance assay” are based on easily determined ...

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