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Author:
Date:
2016-01-20
[Abstract] Damage to plant organs through both biotic and abiotic injury is very common in nature. Arabidopsis thaliana 5-day-old (5-do) seedlings represent an excellent system in which to study plant responses to mechanical wounding, both at the site of the damage and in distal unharmed tissues. Seedlings of wild type, transgenic or mutant lines subjected to single or repetitive cotyledon wounding can be used to quantify morphological alterations (e.g., root length, Gasperini et al., 2015), analyze the dynamics of reporter genes in vivo (Larrieu et al., 2015; Gasperini et al., 2015), follow transcriptional changes by quantitative RT-PCR (Acosta et al., 2013; Gasperini et al., 2015) or examine additional aspects of the wound ...
[摘要] 通过生物和非生物损伤对植物器官的损害在自然界中是非常常见的。拟南芥（Arabidopsis thaliana）5天龄（5-do）幼苗代表了一种优异的系统，其中在损伤部位和远端无害组织中研究植物对机械创伤的反应。经受单次或重复子叶伤害的野生型，转基因或突变品系的幼苗可用于定量形态学改变（例如根长度，Gasperini等人，2015），分析体内报道基因的动力学（Larrieu等人，2015; Gasperini等人，2015），遵循定量的转录变化RT-PCR（Acosta等人，2013; Gasperini等人，2015）或通过大量下游程序检查伤口反应的其它方面。在这里我们说明如何快速，可靠地卷绕年轻幼苗的子叶，并显示两个启动子驱动表达的β-葡萄糖醛酸酶（GUS）在整个幼苗和主根根分生组织，单个或重复子叶伤害后的行为，分别。我们描述了可以容易地适应具体实验需要的两个程序。
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