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Dialysis tubing, high retention seamless cellulose tubing

透析管,高保留无缝纤维素管

Company: Sigma-Aldrich
Catalog#: D0530
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Preparation of Arabinogalactan Glycoproteins from Plant Tissue
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2013-10-05
[Abstract]  This supplements an earlier protocol (Popper, 2011) for the extraction and assay of cell surface arabinogalactan proteins (AGPs). These highly glycosylated glycoproteins (~95% carbohydrate) contain numerous glycomodules with paired glucuronic acid residues that bind Ca2+ in a pH dependent manner (Lamport and Varnai, 2013). Classical AGPs comprise the bulk of cell surface glycoproteins and are thus integral components of a Ca2+ oscillator involved in a signalling pathway where calcium is a “universal signalling currency” analogous to ATP as the universal energy currency. The central role of these peripheral glycoproteins is thus reason enough for their further study. However, problems arise due to the extensive glycosylation and its apparent microheterogeneity ... [摘要]  这补充了用于细胞表面阿拉伯半乳聚糖蛋白(AGP)的提取和测定的早期方案(Popper,2011)。这些高度糖基化的糖蛋白(〜95%碳水化合物)含有许多具有以pH依赖性方式结合Ca 2+ 2+的成对葡萄糖醛酸残基的糖模块(Lamport和Varnai,2013)。经典AGP包含大量细胞表面糖蛋白,因此是参与信号传导途径的Ca 2+ 2+振荡器的整体组分,其中钙是类似于作为通用能量货币的ATP的"通用信号传导货币"。因此,这些外周糖蛋白的中心作用是足以进行进一步研究的理由。然而,由于广泛的糖基化及其明显的微不均一性,通常假定排除简单的还原剂方法而出现问题。在这里,我描述了基于其与β-D-葡糖基或半乳糖基的特异性相互作用的经典AGP的简单部分纯化Yariv试剂,一种合成的重氮染料,其在中性pH的盐溶液中沉淀AGP作为不溶性复合物。 ...

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