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Author:
Date:
2015-12-20
[Abstract] The periderm and exodermis of taproots and tuberous taproots contain an extracellular lipid polymer, suberin, deposited in their cell walls. This polymer is intractable in organic solvents, and is co-deposited with chloroform-extractable waxes. These suberin-associated root waxes are typically composed of alkanes, primary alcohols, fatty acids, alkyl ferulates, alkyl caffeates, and alkyl coumarates (Espelie et al., 1980; Li et al., 2007; Kosma et al., 2015). They are believed to contribute to the diffusion barrier properties of suberized cell walls (Soliday et al., 1979), and possibly have other roles yet to be discovered. Here we describe a protocol to extract and analyze waxes associated with root suberin. This fraction of aliphatic components is ...
[摘要] 子叶和块根状茎的外胚层和外皮含有沉积在其细胞壁中的细胞外脂质聚合物，软骨素。该聚合物在有机溶剂中难以处理，并与氯仿萃取蜡共沉积。这些软骨素相关的根蜡通常由烷烃，伯醇，脂肪酸，烷基阿魏酸酯，烷基咖啡酸酯和烷基香豆酸酯组成（Espelie等人，1980; Li等人，/em>，2007; Kosma ，，2015）。它们被认为有助于增加蜂窝细胞壁的扩散阻挡性能（Soliday等人，1979），并且可能具有尚待发现的其它作用。在这里我们描述一个协议，以提取和分析与根软骨素相关的蜡。该部分脂族组分通过在氯仿中全根浸提提取，然后化学改性以制备更适合于气相色谱分析的样品。该方案针对拟南芥进行优化，但可以与本文所述的其他植物的根一起使用。
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