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2-place Balance for weighing tissues

2位称量纸巾的天平

Company: VWR
Catalog#: 10153-744
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13C Tracer Studies of Metabolism in Mouse Tumor Xenografts
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2015-11-20
[Abstract]  Mice are widely used for human tumor xenograft studies of cancer development and drug efficacy and toxicity. Stable isotope tracing coupled with metabolomic analysis is an emerging approach for assaying metabolic network activity. In mouse models there are several routes of tracer introduction, which have particular advantages and disadvantages that depend on the model and the questions addressed. This protocol describes the bolus i.v. route via repeated tail vein injections of solutions of stable isotope enriched tracers including 13C6-glucose and 13C5,15N2-glutamine. Repeated injections give higher enrichments and over longer labeling periods than a single bolus. Multiple injections of glutamine are necessary to achieve ... [摘要]  小鼠广泛用于癌症发展和药物功效和毒性的人肿瘤异种移植研究。 稳定同位素追踪与代谢物组学分析是一种新兴的方法来测定代谢网络活动。 在小鼠模型中,有几种示踪剂引入途径,其具有取决于模型和所解决的问题的特定优点和缺点。 该协议描述了bolus i.v. 通过重复尾静脉注射稳定的同位素富集的示踪剂的溶液的路径,所述示踪剂包括13 C 13 - 葡萄糖和13 C - 13 C - ,N 15 - ,N 15 - 谷氨酰胺。 与单次推注相比,重复注射给予更高的富集和更长的标记周期。 多次注射谷氨酰胺对于在移植肿瘤中实现充分富集是必需的。

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