cOmpleteTM, Mini, EDTA-free Protease Inhibitor Cocktail
Company: Roche Diagnostics
Catalog#: 04693159001
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Preparation of Crude Synaptosomal Fractions from Mouse Brains and Spinal Cords
Author:
Date:
2017-08-05
[Abstract]  The current protocol describes the preparation of crude synaptosomal fractions from mouse brain or spinal cord samples. In detail, a sequential protocol yielding crude synaptosomal and light membrane fractions is provided. This fast and easy method might be sufficient to assess the amount of synaptic proteins in down-steam applications like Western-blot or ELISA in e.g., mouse models of Alzheimer’s disease or other neurodegenerative conditions.

Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords
Author:
Date:
2017-08-05
[Abstract]  The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are described. This procedure yields soluble and insoluble protein extracts that can be further processed in down-stream applications like ELISA or Western blotting.

Cycloheximide Assays to Measure Protein Degradation in vivo in Plants
Author:
Date:
2016-09-05
[Abstract]  The half-life of a protein is a characteristic property, and can be modulated by post-translational modifications, changes in subcellular localization, and/or interaction with other proteins or ligands. As one determinant of its steady-state level, a protein’s degradation represents an important distinguishing attribute relevant to its biological function. Because protein longevity cannot be elucidated from bioinformatics analyses, it must be determined empirically. Here we describe two ...

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