| In vitro Tumor Cell Migration Assay Using ThinCertsTM (Transwells)
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Author:
Date:
2016-06-05
[Abstract] The high migration rate of tumor cells often results in poor prognosis for the survival of the patients. Here, we describe a protocol to measure the migration of cells using a quantitative assay. The relative tumor cell migration was measured using ThinCertsTM cell culture inserts and a lactate dehydrogenase (LDH) assay to quantify the relative cell number. The quantification of the migration with the LDH kit is much more precise than other methods using i.e. crystal blue to count the cells.
[摘要] 肿瘤细胞的高迁移率通常导致患者的生存的不良预后。 在这里，我们描述了使用定量测定测量细胞迁移的协议。 使用ThinCerts TM细胞培养插入物和乳酸脱氢酶（LDH）测定来测量相对肿瘤细胞迁移以定量相对细胞数。 使用LDH试剂盒的迁移的定量比使用结晶蓝计数细胞的其它方法精确得多。
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| Assay for Adherence of Vibrio cholerae to Eukaryotic Cell Lines
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Author:
Date:
2014-04-20
[Abstract] Vibrio cholerae (V. cholerae) colonizes the intestinal epithelium and secretes cholera toxin (CT), a potent enterotoxin that causes severe fluid loss characteristic of the disease cholera. V. cholerae is a non-invasive Gram-negative bacterium that adheres to intestinal cells as well as a variety of different cell types. A protocol for adherence of V. cholerae to various cell lines is described. The adhered bacteria can be used to examine expression of genes that are differentially expressed between adhered and unadhered bacteria or other purposes (Dey et al., 2013).
[摘要] 霍乱弧菌（霍乱弧菌）殖民肠上皮并分泌霍乱毒素（CT），霍乱毒素（CT）是一种有效的肠毒素，其导致霍乱的特征性严重的流体损失。 V。 霍乱菌是一种非侵入性革兰氏阴性细菌，其粘附于肠细胞以及各种不同的细胞类型。 遵守 V的协议。 霍乱等对各种细胞系的影响。 粘附的细菌可用于检查在粘附和未粘附的细菌或其他目的之间差异表达的基因的表达（Dey等人，2013）。
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| Immunoprecipitation of ROR1
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Author:
Date:
2013-09-20
[Abstract] ROR1 is a receptor tyrosine kinase family member studied for its roles in development and cancer. Here we describe a protocol for immunoprecipitation of endogenous ROR1 from t(1;19) (a disease subtype categorized by its chromosome translocation) acute lymphoblastic leukemia immortalized cell lines.
[摘要] ROR1是受体酪氨酸激酶家族成员，研究其在发展和癌症中的作用。 在这里我们描述了内源性ROR1从t（1; 19）（由其染色体易位分类的疾病亚型）急性淋巴细胞白血病永生化细胞系免疫沉淀的协议。
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