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Glycine, ultra pure

甘氨酸,超纯

Company: Biomol GmbH
Catalog#: 04943.1
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Sample Preparation for Correlative Light and Electron Microscopy (CLEM) Analyses in Cellular Microbiology
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2015-10-05
[Abstract]  Dynamic processes in cells are usually monitored by live cell fluorescence microscopy. Unfortunately, this method lacks the ultrastructural information about the structure of interest (SOI). Currently, electron microscopy (EM) is the best tool to achieve highest spatial resolution. In addition, correlative light and electron microscopy (CLEM) analysis of the same structure allows combining authentic live cell imaging with the resolution power of EM. Additionally the reference space of the SOI is revealed. Our CLEM analyses of HeLa cells allow tracing the morphology and dynamic behavior of intracellular micro-compartments in living cells and their ultrastructure and subcellular organization in a highly resolved manner. [摘要]  通常通过活细胞荧光显微镜监测细胞中的动态过程。 不幸的是,这种方法缺乏关于感兴趣的结构(SOI)的超结构信息。 目前,电子显微镜(EM)是实现最高空间分辨率的最佳工具。 此外,相同结构的相关光和电子显微镜(CLEM)分析允许组合真实活细胞成像与EM的分辨率功率。 此外,揭示了SOI的参考空间。 我们对HeLa细胞的CLEM分析允许以高度解析的方式追踪活细胞中的细胞内微隔室及其超微结构和亚细胞组织的形态和动态行为。

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