| Measuring Secretion of Capsidiol in Leaf Tissues of Nicotiana benthamiana
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Author:
Date:
2018-08-05
[Abstract] Plant species produce a wide variety of antimicrobial metabolites to protect themselves against potential pathogens in natural environments. Phytoalexins are low molecular weight compounds produced by plants in response to attempted attacks of pathogens. Accumulation of phytoalexins in attacked plant tissues can inhibit the growth of penetrating pathogens. Thus phytoalexins play a major role in post-invasion defense against pathogens. Major phytoalexins produced by Solanaceous plants are sesquiterpenoids such as capsidiol produced by Nicotiana and Capsicum species, and rishitin produced by Solanum species, which are synthesized in the cytosol and secreted into the intercellular space of plant tissues. We previously reported that deficiency in capsidiol secretion ...
[摘要] 植物物种产生多种抗微生物代谢物，以保护自身免受自然环境中潜在的病原体的侵害。植物毒素是由植物响应于病原体的企图攻击而产生的低分子量化合物。植物抗毒素在受攻击的植物组织中的积累可以抑制穿透性病原体的生长。因此，植物抗毒素在入侵后对抗病原体的防御中起主要作用。茄科植物产生的主要植物抗毒素是倍半萜类化合物，如由 Nicotiana 和 Capsicum 物种产生的衣壳菌素，以及由 Solanum 物种产生的rishitin，其在胞质溶胶分泌到植物组织的细胞间隙中。我们以前曾报道，衣壳菌素分泌不足会导致本塞姆氏烟草对马铃薯晚疫病菌致病疫霉的敏感性增加。在这里，我们描述了一种测量 N中分泌的衣壳二醇的实用方案。本塞姆氏
【背景】该方案提供了一种快速简便的方法来量化衣壳菌醇的分泌，如Shibata 等（2016）所述。 因为环己烷通常用作洗去花粉层的溶剂，但能保持花粉活力（Doughty et al。，1993），我们开发了这种方法从叶子表面洗去分泌的代谢物来量化细胞外capsidiol。 可以使用该方法分析质膜局部转运蛋白的功能，同时还允许其与其他方法结合使用，例如使用质膜囊泡的生化转运分析（例如，Sugiyama 等人，，2007），以确定所检查的转运蛋白的底物。
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| Detection of Poly (A) RNA in Mesophyll Cells of Nicotiana benthamiana Using in situ Hybridization
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Author:
Date:
2015-09-05
[Abstract] Export of transcribed mRNAs from nucleoplasm to cytosol is an essential process for the translation of genes into proteins. This process is tightly regulated by nuclear pores, composed of about 30 nucleoporin proteins (Nups). Whether or not the mRNAs are able to be appropriately exported to cytoplasm is of an importance for understanding the role of Nups. Here, we describe a practical protocol to detect the intracellular localization of mRNAs in mesophyll cells of Nicotiana benthamiana (N. benthamiana). This protocol is based on poly (A) in situ hybridization method using an oligo d(T) probe conjugated with Alexa Fluor-488.
[摘要] 将转录的mRNA从核质到细胞质的输出是将基因翻译成蛋白质的必要过程。 这个过程受到由约30个核蛋白（Nups）组成的核孔紧密调节。 mRNA是否能够适当地输出到细胞质对于理解Nups的作用是重要的。 在这里，我们描述一种实用的协议，以检测本地烟草的本底细胞中的mRNA的细胞内定位（<本宁顿烟草）。 该方案基于使用与alexa fluor-488缀合的寡聚d（t）探针的poly（a）原位杂交方法。
该方案基于使用与alexa="" fluor-488缀合的寡聚d（t）探针的poly（a）原位杂交方法。="">
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