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Author:
Date:
2015-08-20
[Abstract] In this method, the intestinal lumen of Caenorhabditis elegans (C. elegans) is labeled with a fluorescent fluid-phase marker, Texas Red-dextran. Since dextran conjugates are membrane impermeable, animals fed with it show a red fluorescent signal in the lumen of the intestine. Texas Red-dextran in the lumen is not efficiently endocytosed by intestinal cells and is not effectively transported to the body cavity paracellularly. It is useful to determine whether round-shaped membrane structures are invaginations from the apical membrane or cytoplasmic vesicles. If the barrier function of the intestinal epithelium is impaired, Texas Red-dextran can leak from the intestinal lumen to the body cavity. Therefore, this method can be used to visualize apical membrane morphology in ...
[摘要] 在这种方法中，秀丽隐杆线虫（秀丽隐杆线虫）的肠腔内用荧光液相标记德克萨斯红葡聚糖标记。 由于葡聚糖结合物是不透膜的，所以喂食的动物在肠腔中显示出红色荧光信号。 德克萨斯州的红葡萄糖在管腔中不能有效地被肠细胞内吞，并且不能被有效地输送到细胞外的体腔。 确定圆形膜结构是否是从顶端膜或细胞质囊泡的入内是有用的。 如果肠上皮的屏障功能受损，德克萨斯红葡聚糖可以从肠腔泄漏到体腔。 因此，该方法可用于显示肠细胞中的顶端膜形态，并研究肠上皮的阻隔性。
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