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Author:
Date:
2012-02-20
[Abstract] Small Interfering RNA (siRNA) is a class of double-stranded RNAs of 20-25 nucleotides that play important roles in many biological processes (Hamilton and Baulcombe, 1999). siRNAs act by “neutralizing” the mRNA of the target protein, facilitating degradation of the mRNA and hence altering the biological effect of the protein (reviewed in Hannon and Rossi, 2004). siRNAs may also change the intracellular levels of regulatory RNAs. Use of siRNAs for manipulating the expression of genes of interest in biological research is commonly referred to as RNA interference or knockdown technique (Elbashir et al., 2001). Synthetic siRNAs are an emerging tool that are now widely used in these studies. A variety of algorithms are employed by different companies for the design of siRNA products, ...
[摘要] 小干扰RNA（siRNA）是一类20-25个核苷酸的双链RNA，在许多生物过程中起重要作用（Hamilton和Baulcombe，1999）。 siRNA通过"中和"靶蛋白的mRNA起作用，促进mRNA的降解，从而改变蛋白的生物效应（综述于Hannon和Rossi，2004）。 siRNA也可以改变调节RNA的细胞内水平。 在生物研究中使用siRNA操纵目的基因的表达通常被称为RNA干扰或敲除技术（Elbashir等人，2001）。 合成的siRNA是一种新兴的工具，现在广泛应用于这些研究。 不同公司采用多种算法来设计siRNA产品，其在功效，特异性和成本等方面不同。 在此使用来自Dharmacon的siGENOME SMARTpool试剂解释siRNA敲低的示例方案。
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