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DR4 MEF Feeder Cells, P3, irradiated

DR4 MEF饲养细胞,P3,照射

Company: Applied StemCell
Catalog#: ASF-1013
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Culturing of C57BL/6 Mouse Embryonic Stem (ES) Cell Line
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2011-10-05
[Abstract]  Using ATCC ES C57BL/6 as an example, it is shown here how to culture mouse embryonic stem (ES) cell line. The clonal embryonic stem cell line #693 ES C57BL/6 was derived from a strain C57BL/6J (B6) mouse blastocyst [PubMed: 11730008]. The ES cells were shown to populate the germ line of two host blastocyst donors, FVB/NJ (FVB) and the coisogenic strain C57BL/6-Tyrc-2J (c2J). Coat-color chimera production was high using c2J blastocysts while FVB blastocysts produced a low number of chimeras [PubMed: 11730008]. [摘要]  使用ATCC ES C57BL/6作为实例,在此显示如何培养小鼠胚胎干(ES)细胞系。 克隆胚胎干细胞系#693ES C57BL/6源自菌株C57BL/6J(B6)小鼠胚泡[PubMed:11730008]。 ES细胞显示为填充两个宿主胚泡供体FVB/NJ(FVB)和同种型菌株C57BL/6-Tyrc-2J(c2J)的种系。 使用c2J胚泡的外套嵌合体产量高,而FVB胚泡产生低数量的嵌合体[PubMed:11730008]。

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