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2021-01-20
[Abstract] The synapse is a complex structure where the transmission of information takes place. Synaptic dysfunction is one of the earliest pathophysiological events in several diseases, such as traumatic brain injury, cerebral ischemia, and neurodegenerative diseases. Thus, a methodology to study synaptic structure and function is crucial for the development of potential strategies for the treatment of many neurological diseases. Synaptoneurosomes (SNs) are structures assembled by the sealed presynaptic bouton and the attached post-synaptic density. Despite the fact that for a long time it has been recognized that SNs are a powerful tool to study synaptic function, composition, and structure, its use has been limited by the requirement of relatively large amounts of material to successfully ...
[摘要] [摘要]突触是一个复杂的结构,在此结构中发生信息的传递。小号ynaptic功能障碍是几种疾病,如脑外伤,脑缺血最早的病理生理活动之一,和神经退行性疾病。因此,研究突触结构和功能的方法学对于开发治疗许多神经系统疾病的潜在策略至关重要。突触神经小体(SN)是由密封的突触前钮扣和附加的突触后密度组装而成的结构。尽管很长一段时间以来,人们已经认识到SN是研究突触功能,组成的强大工具,在结构和结构上,其使用受到成功地隔离它们所需的相对大量材料的限制。在这里,我们描述了在低渗条件下执行的三步离心程序,以从少量大脑皮层中分离出SN。
图形摘要:
突触神经体的制备流程图。
[背景]突触是由连接至突触后终端和突触前布顿组装的结构所包围由星形细胞伸长率(Halassa等人,2007 )。在过去的十年中,见证了许多用于研究其结构和功能的高度复杂的技术的发展。但是,SN仍被大量研究者所使用,因为它们相对容易分离并产生有关突触结构和功能的有价值的信息。SNs的制备是在特定的低渗条件下进行的改良的细胞分离程序,可分离突触前突突和附着的突触后元件。在鉴定的最初阶段,它们被称为无细胞响应制剂,因为它们对激素具有响应(Chasin等,197 4 ;Horn和Phillipson ,1976 ...
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