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Anti-Human/Mouse CD45R (B220) Biotin

抗人/小鼠CD45R(B220)生物素

Company: Thermo Fisher Scientific
Catalog#: 13-0452
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Isolation of ILC2 from Mouse Liver
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Date:
2014-07-20
[Abstract]  Group 2 innate lymphoid cells (ILC2) are a recently characterized cell population which lacks specific antigen receptors and contributes to immune responses at mucosal surfaces of lung and gut. Recently, we demonstrated that ILC2 expand in the context of chronic liver diseases in mice and contribute to pathology in an IL-33 dependent manner. Here, we describe a protocol to isolate highly purified ILC2 from mouse livers. This procedure provides effective digestion of liver tissue and limits proteolytic degradation of cell surface receptors leading to increased yields of biologically functional cells. The number of liver resident ILC2 in the steady state is low, however their number dramatically increase upon systemic or local treatment of mice with cytokines such as IL-25 and IL-33. Using ... [摘要]  组2天然淋巴样细胞(ILC2)是最近表征的细胞群体,其缺乏特异性抗原受体并且有助于在肺和肠的粘膜表面的免疫应答。 最近,我们表明ILC2扩展在小鼠慢性肝病的背景下,并有助于病理学在IL-33依赖的方式。 在这里,我们描述了从小鼠肝脏分离高度纯化的ILC2的协议。 该方法提供肝组织的有效消化并限制细胞表面受体的蛋白水解降解,导致生物功能细胞的产量增加。 在稳定状态下肝脏驻留的ILC2的数量低,然而,在用细胞因子例如IL-25和IL-33全身或局部治疗小鼠时,它们的数量显着增加。 使用基于小环的表达构建体用于这些细胞因子,大量的功能性ILC2可以用本文提供的方案分离。

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