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Author:
Date:
2014-07-05
[Abstract] Pulse-chase method is a powerful technique used to follow the dynamics of proteins over a period of time. The expression level, processing, transport, secretion or half-life of proteins can be tracked by metabolically labeling the cells, such as with radiolabeled amino acids (pulse step). This protocol describes the condition used to study the folding and disulfide bond formation of immunoglobulin in suspension cells. With some minor modifications, this protocol can be adapted to study the degradation rate or the secretion of target proteins.
[摘要] 脉冲追踪法是用于跟踪蛋白质在一段时间内的动力学的有力技术。 蛋白质的表达水平，加工，转运，分泌或半衰期可以通过代谢标记细胞，例如用放射性标记的氨基酸（脉冲步骤）来追踪。 该方案描述了用于研究悬浮细胞中免疫球蛋白的折叠和二硫键形成的条件。 通过一些微小的修改，这个协议可以适应于研究目标蛋白的降解速率或分泌。
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