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Author:
Date:
2013-08-05
[Abstract] Plants have developed two distinct mechanisms, i.e., strategy I (reduction strategy) and II (chelation strategy), to mobilize insoluble Fe(III) in the rhizosphere and transport it through the plasma membrane. Arabidopsis thaliana and other dicots rely on strategy I. In this strategy, the rhizosphere is first acidified by a PM-localized H+-ATPase, AHA2. Then, FERRIC CHELATE REDUCTASE 2 (FRO2) reduces Fe(III) to soluble Fe(II). Finally, the reduced Fe is taken up by a high-affinity transporter, IRON-REGULATED TRANSPORTER 1 (IRT1). Root ferric chelate reductase activity can be quantified spectrophotometrically by the formation of Purple-colored Fe(II)-ferrozine complex in darkness.
[摘要] 植物已经开发了两种不同的机制，即，策略I（还原策略）和II（螯合策略），以在根际中移动不溶性Fe（III）并将其运输通过质膜。 拟南芥和其它双子叶植物依赖策略I.在该策略中，根际首先通过PM局部化H + -ATP酶AHA2酸化。 然后，FERRIC CHELATE REDUCTASE 2（FRO2）将Fe（III）还原为可溶性Fe（II）。 最后，还原的Fe被高亲和性转运蛋白IRON-REGULATED TRANSPORTER 1（IRT1）摄取。 根铁螯合物还原酶活性可以通过分光光度法通过在黑暗中形成紫色Fe（II） - ferrozine复合物来定量。
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