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Author:
Date:
2014-06-20
[Abstract] The protoplasts assay constitutes a powerful tool that allows an easy uptake of active agents and a precise quantification of cell death induction in different populations. Our study showed that the basal level of cell death in our controls is low and stable throughout the length of our experiments (Danon et al., 2005; Pineau et al., 2013). In addition, the data obtained from the protoplast assay are applicable to intact seedlings, where it is possible to see differences in the intensity of necrotic lesions (Danon et al., 2006) even if those differences are not as easily and clearly quantifiable as with the protoplast assay.
[摘要] 原生质体测定构成了一种强有力的工具，其允许活性剂的容易摄取和在不同群体中细胞死亡诱导的精确量化。 我们的研究表明，我们的对照中的细胞死亡的基础水平低并且在整个实验的长度上是稳定的（Danon等人，2005; Pineau等人， 2013）。 此外，从原生质体测定获得的数据适用于完整的幼苗，其中可以看到坏死损伤强度的差异（Danon等人，2006），即使这些差异不是 如同原生质体测定一样容易和清楚地定量。
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