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Author:
Date:
2014-04-20
[Abstract] Reductive dehalogenation has been found primarily in anaerobic communities and is originally thought to rarely occur in aerobes. A reductive dehalogenase (BhbA) was characterized from an aerobic strain of Comamonas sp. 7D-2, which was isolated from a bromoxynil octanoate-contaminated soil sample collected in Jiangsu, China. BhbA catalyzes the reductive dehalogenation of bromoxynil and its derivative 3,5-dibromo-4-hydroxybenzoate under aerobic conditions. BhbA is membrane-associated and found to have the key features of anaerobic respiratory reductive dehalogenases. This protocol describes the method for enzyme analysis of the aerobic reductive dehalogenase (BhbA) in the membrane fraction.
[摘要] 还原脱卤主要在厌氧社区中发现，并且最初被认为很少发生在aerobes中。 还原脱卤素酶（BhbA）的特征在于Comamonas的需氧菌株。 7D-2，其从中国江苏收集的溴苯腈辛酸盐污染的土壤样品中分离。 BhbA在需氧条件下催化溴苯腈及其衍生物3,5-二溴-4-羟基苯甲酸的还原脱卤。 BhbA是膜相关的并且发现具有厌氧呼吸还原脱卤素酶的关键特征。 该方案描述了膜部分中有氧还原脱卤酶（BhbA）的酶分析方法。
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