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Author:
Date:
2014-04-05
[Abstract] The intestinal lamina propria contains a dense network of T cells, dendritic cells (DCs) and macrophages, which play an important role in local innate and adaptive immune responses. We have recently identified distinct subsets of DCs (Persson et al., 2013) and macrophages (Bain et al., 2013) in the human intestine. In addition, we have studied T cells in healthy and diseased intestine. Here, we describe two methods for isolating these cell populations: 1) enzymatic treatment and 2) migration based isolation. The enzymatic method can be used to isolate T cells, DC and macrophages, whereas the migration based ‘walk-out’ protocol is suitable for DC isolation, as these cells migrate out from the tissues.
[摘要] 肠固有层含有T细胞，树突细胞（DC）和巨噬细胞的密集网络，其在局部先天和适应性免疫应答中起重要作用。 我们最近在人肠中鉴定了不同的DC亚群（Persson等人，2013）和巨噬细胞亚群（Bain等人，2013）。 此外，我们研究了健康和患病肠中的T细胞。 在这里，我们描述了两种方法隔离这些细胞群体：1）酶处理和2）基于迁移的隔离。 酶法可用于分离T细胞，DC和巨噬细胞，而基于迁移的"走出"方案适合于DC分离，因为这些细胞从组织迁出。
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