|
Author:
Date:
2016-03-05
[Abstract] Plant regeneration refers to a process through which an explant is differentiated to a whole plant under certain conditions. It has been shown that two plant hormones, auxin and cytokinin, play critical roles within this process (Duclercq et al., 2011). Cytokinin induces shoot regeneration, whereas auxin promotes root production. In addition to hormones, recent study has revealed that age cue serves as a common element behind plant cell totipotency (Toledano et al., 2012). Here we present an easy protocol to assess the shoot regenerative capacity of Arabidopsis and tobacco leaves of different ages.
[摘要] 植物再生是指在某些条件下外植体分化成整株植物的过程。 已经表明，两种植物激素，植物生长素和细胞分裂素在该过程中起关键作用（Duclercq等人，2011）。 细胞分裂素诱导芽再生，而生长素促进根生产。 除了激素，最近的研究已经揭示年龄提示作为植物细胞全能性背后的共同元素（Toledano等人，2012）。 在这里我们提出了一个简单的协议，以评估不同年龄的拟南芥和烟草叶的射线再生能力。
|