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Company: Thermo Fisher Scientific

Catalog#: 11090-08

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Isolation and Enrichment of Major Primary Neuroglial Cells from Neonatal Mouse Brain

Santosh Kumar Samal, Madhav Sharma and Jayasri Das Sarma

Published online: 2024-01-20

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Midbrain Neuron-glia Mixed Cultures

Author:

Huiming Gao,

Date:

2012-04-05

[Abstract] Mixed neuron-glia cultures provide a unique tool to study cellular contribution and molecular pathways in various neurological disorders. They are also invaluable for exploring neuron-glia interaction under physiological and pathological conditions. The relatively long-lasting midbrain neuron-glia mixed cultures generated following this protocol have been widely used to study the pathogenesis of Parkinson’s disease, the most common neurodegenerative movement disorder. [摘要] 混合神经元-神经胶质细胞培养提供了一个独特的工具来研究细胞的构成和各种神经系统疾病的分子途径。他们在探索生理和病理条件下神经元-胶质细胞相互作用方面大有用处。按照操作指南上相对持久的中脑的神经元—神经胶质细胞混合培养已被广泛用于研究帕金森氏症——最常见的神经性运动障碍疾病的发病机制。这份指南经过了Dr. Hong实验室不同研究人员尤其是Dr. Bin Liu多年的改进。

Generation of Neuron-enriched Cultures (Method 1)

Author:

Huiming Gao,

Date:

2011-11-05

[Abstract] This protocol will guide you through the process for generating midbrain neuronal cultures from late embryo mouse brain. These cultures serve as a useful tool to study molecular pathways during neuronal death in various neurological disorders. This method uses cytosine β-D-arabinofuranoside in the cultures to suppress the proliferation of glial cells. Seven days after the seeding, the neuron-enriched cultures prepared following this protocol will contain less than 10% glia cultures. The concentrations of β-D-arabinofuranoside should be adjusted according to your culture condition. This protocol has been developed and improved over the years by various researchers in Dr. Hong’s lab, especially Dr. Bin Liu. [摘要] 这个协议将指导您通过从晚胚胎小鼠大脑产生中脑神经元文化的过程。这些文化作为一个有用的工具，研究神经元死亡期间各种神经系统疾病的分子途径。该方法在培养物中使用胞嘧啶β-D-阿拉伯呋喃糖苷抑制神经胶质细胞的增殖。接种后7天，按照该方案制备的富含神经元的培养物将含有少于10％的胶质细胞培养物。应根据您的培养条件调整β-D-阿拉伯呋喃糖苷的浓度。这个协议已经由Hong博士实验室的各种研究人员，尤其是刘博士多年来开发和改进。

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