| Mouse CD8+ T Cell Migration in vitro and CXCR3 Internalization Assays
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Author:
Date:
2017-03-20
[Abstract] Chemokines are molecules that regulate the positioning of cells during homeostasis and inflammation. CXCL10 is an interferon-induced chemokine that attracts cells that express the chemokine receptor CXCR3 on their surface. CXCL10 expression is often induced upon inflammation and guides lymphocytes, such as T and NK cells, into the injured tissues. Notably, CXCL10 binding to CXCR3 induces receptor internalization and, therefore, low CXCR3 levels in cells positive for CXCR3 expression can be indicative of chemokine signaling.
Here, we describe an in vitro method to evaluate the ability of murine CD8+ T cells to migrate towards recombinant murine CXCL10; and a flow cytometry assay to measure CXCR3 expression levels at the surface of T cells, after exposure to ...
[摘要] 趋化因子是调节体内平衡和炎症期间细胞定位的分子。 CXCL10是干扰素诱导的趋化因子，其吸引在其表面上表达趋化因子受体CXCR3的细胞。 CXCL10表达通常在炎症诱导并引导淋巴细胞如T和NK细胞进入受损组织。值得注意的是，CXCL10与CXCR3结合诱导受体内化，因此CXCR3表达阳性细胞中的CXCR3水平降低可能是趋化因子信号传导的指示。
＆nbsp;这里，我们描述体外方法来评估鼠CD8 + T细胞向重组鼠CXCL10迁移的能力;以及暴露于不同剂量的趋化因子后在T细胞表面测量CXCR3表达水平的流式细胞术测定。
背景 趋化因子介导的T细胞运输是稳态和炎症期间的重要过程。活化的CD8 + T细胞表达趋化因子受体，例如CXCR3，允许它们向趋化因子CXCL9,10和11迁移，通常在损伤组织上上调。调节T细胞迁移的分子线索的评估对于了解其功能背后的生物学非常重要，但是在体内运行的复杂机制有时难以去卷积。在这里，我们提供有关体外方法的详细信息，以评估CD8 + T细胞上的趋化因子功能，重点是CXCL10介导的化学吸引和CXCR3内化。我们使用可以容易地在体外扩增和活化的抗原特异性转基因CD8 +细胞，因此提供足够数量的表型相同的淋巴细胞（例如， ...
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| Isolation of CD34+ Cells from Human Fetal Liver and Cord Blood
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Author:
Date:
2013-12-05
[Abstract] CD34 is a glycosylated cell surface protein and represents a well-known marker for primitive progenitor cells in various organs, especially cord blood, bone marrow and fetal liver. CD34+ progenitor cells are suitable for a series of studies, e.g. cell differentiation, transplantation as well as construction of humanized mouse models. Here, we describe a method to isolate CD34+ cells from the human cord blood and fetal liver.
[摘要] CD34是糖基化的细胞表面蛋白，并且代表了在各种器官，特别是脐带血，骨髓和胎儿肝脏中的原始祖细胞的公知标记。 CD34 +祖细胞适合于一系列研究，例如细胞分化，移植以及人源化小鼠模型的构建。 在这里，我们描述了一种从人脐带血和胎儿肝脏中分离CD34 - sup + +细胞的方法。
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