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CASY Cell Counter Model TT

CASY细胞计数器模型TT

Company: Roche Diagnostics
Catalog#: 05651735001
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Immunoblot Analysis of Histone H4 Acetylation and Histone H2A Phosphorylation in Candida albicans
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Date:
2013-10-20
[Abstract]  Posttranslational modifications of histones are required for different processes including transcription, replication and DNA damage repair. This protocol describes the preparation of a whole-cell extracts for the fungal pathogen Candida albicans. Furthermore, the extract is used to detect lysine acetylation of histone H4 as well as serine 129 phosphorylation of histone H2A by immunoblot analysis.
[摘要]  包括转录,复制和DNA损伤修复的不同过程需要组蛋白的翻译后修饰。 该方案描述了真菌病原体白色念珠菌的全细胞提取物的制备。 此外,提取物用于通过免疫印迹分析检测组蛋白H4的赖氨酸乙酰化以及组蛋白H2A的丝氨酸129磷酸化。

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