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Author:
Date:
2013-09-05
[Abstract] Thymidine Kinase from human Herpes simplex virus type 1 (HSV1-TK) in combination with specific substrate prodrug nucleotide analogue ganciclovir (GCV) has been widely used as suicidal therapeutic gene for cancer gene therapy. HSV1, and its mutant (HSV1-sr39TK) with improved substrate specificity, were used as reporter genes for PET-imaging of various biological functions in small animals, by combining with radiolabeled substrates such as 18F-FHBG and 124I-FIAU. 3H-Penciclovir (PCV) uptake assay is a method of choice used to determine the expression level of HSV1-TK in mammalian cells and tissues. HSV1-TK phosphorylate PCV and result in the formation of penciclovir monophosphate, and its subsequent phopsphorylation by cellular TK lead to ...
[摘要] 来自人单纯疱疹病毒1型（HSV1-TK）的胸苷激酶与特定底物前药核苷酸类似物更昔洛韦（GCV）的组合已广泛用作癌症基因治疗的自杀性治疗基因。将具有改善的底物特异性的HSV1和其突变体（ HSV1-sr39TK ）用作小动物中各种生物学功能的PET成像的报告基因，通过与放射性标记的底物例如18 F-FHBG和124 I-FIAU。 H-Penciclovir（PCV）摄取试验是一种选择用于测定哺乳动物细胞和组织中HSV1-TK表达水平的方法。 HSV1-TK磷酸化PCV并导致形成penciclovir单磷酸，并且其随后通过细胞TK的磷酸化导致形成选择性地捕获在表达HSV-TK的细胞中的三磷酸喷昔洛韦。通过诸如闪烁计数的放射性方法，可以检测到更昔洛韦对哺乳动物细胞和组织的摄取。在这里我们描述在哺乳动物细胞中进行 H - Penciclovir摄取的方案。
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