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Sephadex G-50 column

illustra MicroSpin G-50柱

Company: Cytiva
Catalog#: 27-5330-01
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32P Radioactive Probe Synthesis and Preparation
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Date:
2011-12-20
[Abstract]   To probe for a specific mRNA species by Northern blot, RNA from the agarose/formaldehyde gel needs to be transferred to a nylon membrane. RNA is detected by hybridization using a labeled probe. The probe is a DNA or RNA molecule that is chemically or radioactively labeled. In this protocol synthesis and preparation of a [32P]-dCTP-labeled probe is described. [摘要]  为了通过Northern印迹探测特定的mRNA种类,来自琼脂糖/甲醛凝胶的RNA需要转移到尼龙膜上。 使用标记的探针通过杂交来检测RNA。 探针是化学或放射性标记的DNA或RNA分子。 在该方案中,描述了[32 P] -dCTP标记的探针的合成和制备。

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