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Brefeldin A

Brefeldin A

Company: Sigma-Aldrich
Catalog#: B6542
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Whole Spleen Flow Cytometry Assay
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2013-08-05
[Abstract]  In the Whole Spleen Flow Cytometry Assay, we used splenocytes directly ex vivo for stimulation with a variety of TLR ligands. The splenocytes were stimulated for a total of 4 hours, then stained for intracellular cytokines. We then examined cytokine production via flow cytometry. This allowed us to compare the responses of minimally manipulated primary macrophages/monocytes and conventional dendritic cells. [摘要]  在全脾流式细胞术测定中,我们直接使用脾细胞离体用各种TLR配体刺激。 将脾细胞刺激总共4小时,然后对细胞内细胞因子染色。 然后我们通过流式细胞术检查细胞因子产生。 这使我们能够比较最小操纵的原代巨噬细胞/单核细胞和常规树突状细胞的反应。

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