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Author:
Date:
2013-07-20
[Abstract] Cyclic di-GMP (c-di-GMP) has emerged as an important intracellular signaling molecule, controlling the transitions between planktonic (free-living) and sessile lifestyles, biofilm formation, and virulence in a wide variety of microorganisms. The following protocol describes the extraction and quantification of c-di-GMP from Pseudomonas aeruginosa samples. We have made every effort to keep the protocol as general as possible to enable the procedure to be applicable for the analysis of c-di-GMP levels in various bacterial species. However, some modifications may be required for the analysis of c-di-GMP levels in other bacterial species.
[摘要] 环状di-GMP（c-di-GMP）已经作为重要的细胞内信号传导分子出现，控制在各种微生物中浮游生物（自由生命）和固着的生活方式，生物膜形成和毒力之间的转变。 以下方案描述了从绿脓假单胞菌样品中提取和定量c-di-GMP。 我们已尽一切努力使方案尽可能一般，以使该程序适用于分析各种细菌物种中的c-di-GMP水平。 然而，对于其他细菌物种中c-di-GMP水平的分析可能需要一些修饰。
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