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10% fetal bovine serum (FBS)

10%胎牛血清(FBS)

Company: Cytiva
Catalog#: sv143-03
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Endosomal pH Measurement in Bone Marrow Derived Dendritic Cells
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Date:
2013-07-20
[Abstract]  Endosomes embraces different set of compartments such as early endosomes, intermediate endosomes and late endosomes or lysosomes. They become acidic as they mature. This acidification is generated by the vacuolar membrane proton pump V-ATPase that is recruited in late endosomes. This protocol described the measurement of endosomal pH using dextran molecules labelled with pH sensitive and insensitive dyes.
[摘要]  内体包含不同组的区室,例如早期内体,中间内体和晚期内体或溶酶体。 它们成熟时变成酸性。 这种酸化由在后期内体中招募的液泡膜质子泵V-ATP酶产生。 该协议描述了使用用pH敏感和不敏感染料标记的葡聚糖分子测量内体pH。

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