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Alphascreen® cAMP Assay Kit

Alphascreen ® cAMP Assay Kit

Company: PerkinElmer
Catalog#: 6760625
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cAMP Accumulation Assays Using the AlphaScreen® Kit (PerkinElmer)
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2013-07-05
[Abstract]  Cyclic adenosine monophosphate (cAMP) is an intracellular signaling messenger derived from the catalytic conversion of ATP, and is a major product of activated Gs protein-coupled receptors. Conversely, formation of cAMP is inhibited by Gi protein-coupled receptors. This protocol has been optimized for the detection of ligand-mediated cAMP accumulation in adherent immortal cell lines expressing Gs-coupled receptors. [摘要]  环腺苷一磷酸(cAMP)是来源于ATP的催化转化的细胞内信号传导信号,并且是活化的G蛋白偶联受体的主要产物。 相反,cAMP的形成受G 1 i蛋白偶联受体抑制。 该方案已经被优化用于检测表达G s - 偶联受体的贴壁无限增殖细胞系中配体介导的cAMP积累。

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