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Author:
Date:
2012-09-05
[Abstract] Polyadenylation is a post-transcriptional modification of RNA occurring in prokaryotes, eukaryotes and organelles. However, the function and extent of bacterial polyadenylation are in marked contrast to those of eukaryotic poly(A) tails. In fact, the long poly(A) tails of eukaryotic mRNAs play an important role in their exportation to the cytoplasm and promote mRNA stability and translation, whereas the short bacterial tails facilitate RNA decay. One of the obstacles encountered by investigators studying bacterial polyadenylation is the scarcity of polyadenylated RNAs. The method described here allows reverse transcription and PCR amplification of the whole population of polyadenylated RNAs provided that the poly(A) tails are long enough to hybridize to oligo dT30 sequence. To this end ...
[摘要] 多腺苷酸化是在原核生物，真核生物和细胞器中发生的RNA的转录后修饰。 然而，细菌聚腺苷酸化的功能和程度与真核多聚腺苷酸尾部的功能和程度形成鲜明对比。 事实上，真核mRNA的长聚（A）尾部在它们输出到细胞质中起重要作用，并且促进mRNA稳定性和翻译，而短的细菌尾部促进RNA衰变。 研究细菌聚腺苷酸化的研究者遇到的障碍之一是聚腺苷酸化RNA的缺乏。 本文所述的方法允许对聚腺苷酸化的RNA的整个群体进行逆转录和PCR扩增，条件是聚（A）尾足够长以与oligo dT30序列杂交。 为此，使用核酸外切酶缺陷菌株可能是有用的。
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