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Author:
Date:
2015-09-20
[Abstract] Indirect calorimeter is a powerful tool to monitor resting energy metabolism through the measurement of oxygen (O2) consumption and carbon dioxide (CO2) production. From the measurement of VO2 and VCO2, the respiratory exchange ratio (RER) can be calculated to assess energy fuel utilization and energy expenditure (Evan et al., 2012). Previously, indirect calorimeter has been widely used in metabolic disease research in mice to reveal the potential roles of specific genes or treatments in regulating energy metabolism (for example: Bi et al., 2014; Feng et al., 2014). Here, we described a protocol to evaluate the resting energy metabolism of C57BL/6 mice during dark and light cycles using the Oxymax Open Circuit indirect ...
[摘要] 间接热量计是通过测量氧（O 2）消耗和二氧化碳（CO 2）生产来监测静息能量代谢的有效工具。 根据VO 2和VCO 2的测量，可以计算呼吸交换比（RER）以评估能量燃料利用和能量消耗（Evan et al。 ，2012）。 以前，间接热量计已经广泛用于小鼠的代谢疾病研究中，以揭示特定基因或治疗在调节能量代谢中的潜在作用（例如：Bi等人，2014; et al。，2014）。 在这里，我们描述了一个协议，以评估C57BL/6小鼠的休息能量代谢在黑暗和光周期使用Oxymax开路间接量热仪。
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