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Author:
Date:
2015-03-20
[Abstract] The protocol describes the procedure of total RNA isolation from cells of the cyanobacterium Synechocystis sp. PCC 6803. This protocol is also applicable to Synechococcus elongatus PCC 7942 and PCC 6301, Thermosynechococcus vulcanus, and other unicellular and filamentous species of cyanobacteria that do not have thick polysaccharide-containing outer layers. For the latter, Trizol-containing protocols should be adapted. The yield of RNA depends on optical density of cyanobacterial culture and may reach up to 10-20 µg of total RNA per 1 ml of cell culture. RNA isolated by this method can be used for Northern blot hybridization, RT-qPCR, microarrays and Next Generation Sequencing.
[摘要] 该方案描述了从蓝细菌集胞藻的细胞总RNA分离的程序。 PCC 6803。该方案也适用于细长聚球藻PCC 7942和PCC 6301，硫酸高热杆菌（Thermosynechococcus vulcanus）和其它单细胞和丝状物种的蓝细菌，其不具有含多糖的 外层。 对于后者，应该修改含有Trizol的协议。 RNA的产量取决于蓝细菌培养物的光密度，并且可以达到每1ml细胞培养物高达10-20μg的总RNA。 通过该方法分离的RNA可用于Northern印迹杂交，RT-qPCR，微阵列和下一代测序。
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