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Author:
Date:
2013-09-20
[Abstract] Here we describe procedures for the flower cuticular waxes extraction, modification and subsequent qualitative and quantitative analysis by gas-chromotography-mass spectrometry (GC-MS) and gas-chromotography with flame ionization detector (GC-FID), accordingly. To characterize flower cutin monomers two experimental setup are described: (i) analysis of enzymatically isolated cuticles in order to determine the relative proportions of cutin monomers; (ii) analysis of freeze-dried material for quantitative estimation of the cutin content. This report is an adaptation of the earlier published protocols developed for the chemical analysis of the cuticles in vegetative organs (Leide et al., 2007).
[摘要] 在这里我们介绍花角质蜡提取，修改和随后的气相色谱 - 质谱（GC-MS）和气相色谱与火焰离子化检测器（GC-FID）的定性和定量分析的程序。 为了表征花角质单体，描述了两个实验装置：（i）分析酶分离的角质层，以确定角质单体的相对比例; （ii）冷冻干燥材料的分析，用于定量估计角质含量。 该报告是对早期公开的用于营养器官中角质层的化学分析的协议的改编（Leide等人，2007）。
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