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1x Trypsin-EDTA (0.05% / 0.02% in PBS)

1x胰蛋白酶-EDTA(0.05%/ 0.02%在PBS中)

Company: GE Healthcare
Catalog#: L11-004
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Measurement of IFN-α Subtype Concentrations (Virus-free, Cell-based Bioassay)
Author:
Date:
2013-06-20
[Abstract]  The induction of type I IFN is the immediate host response against viral infections. Type I IFNs belong to a multigene family including up to 14 different IFN-α subtypes and one IFN-β. They are highly conserved and bind the same receptor (IFNAR1/2) with varying affinities, although they differ in their biological activities. [摘要]  I型IFN的诱导是针对病毒感染的直接宿主应答。 I型IFNs属于多基因家族,包括多达14种不同的IFN-α亚型和一种IFN-β。 它们是高度保守的,并以不同的亲和力结合相同的受体(IFNAR1/2),尽管它们的生物活性不同。

Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA)
Author:
Date:
2013-01-05
[Abstract]  Reactive oxygen species (ROS) play a critical role in cellular physiopathology. ROS are implicated in cell proliferation, signaling pathways, oxidative defense mechanisms responsible for killing of bacteria, thyroid hormonosynthesis, etc. The cellular Redox homeostasis is balanced by oxidants and antioxidants systems. In several diseases (cancer, neurodegenerative diseases, cardiovascular diseases), the Redox balance is disturbed. In fact, excessive amounts of ROS can damage proteins, lipids and DNA at cellular level.

The choose of a sensitive method for detection of intracellular ROS is very important for detecting the disturbed Redox balance in pathological cells and after exposition of cells to different genotoxic agents (Irradiation, Oxidative stress, etc).

The ...
[摘要]  活性氧(ROS)在细胞病理生理学中起关键作用。 ROS涉及细胞增殖,信号通路,负责杀死细菌的氧化防御机制,甲状腺激素合成等。细胞氧化还原稳态由氧化剂和抗氧化剂系统平衡。在几种疾病(癌症,神经变性疾病,心血管疾病)中,氧化还原平衡受到干扰。事实上,过量的ROS可以在细胞水平损伤蛋白质,脂质和DNA。
选择用于检测细胞内ROS的灵敏方法对于检测病理细胞中和在将细胞暴露于不同基因毒性剂(辐射,氧化应激等)之后的干扰的氧化还原平衡是非常重要的。在生物系统中检测ROS是困难的几个原因:方法敏感性和探针特异性。通常使用2',7'-二氯二氢荧光素二乙酸酯(H 2 DCF-DA)荧光探针,并且可与几种ROS反应,包括过氧化氢,羟基和过氧亚硝酸酯。细胞渗透性H 2 DCFDA被动扩散到细胞中,并在细胞内酯酶切割后保留在细胞内水平。在被ROS氧化时,非荧光H 2 DCFDA转化为高度荧光的2',7'-二氯荧光素(DCF)。 H H 2 DCFDA(CM-H 2 DCFDA)的氯甲基衍生物在活细胞中比H H 2 DCFDA提供好得多的保留。死亡或死亡细胞产生ROS。当我们想检测活细胞中的ROS时,我们必须通过碘化丙啶(PI)染色细胞,并仅在PI阴性的活细胞中评估ROS产生。事实上,PI插入到双链核酸中。它被活细胞排除,但可以穿透死亡或死亡细胞的细胞膜。 ...

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