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NE-PERTM Nuclear and Cytoplasmic Extraction Reagents

NE-PER TM核和细胞质提取试剂

Company: Thermo Fisher Scientific
Catalog#: 78835
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A Protocol for Electrophoretic Mobility Shift Assay (EMSA) from Primary Neuron
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2012-12-05
[Abstract]  The interaction of transcriptional or co-transcriptional factors with DNA is crucial for changes of neuronal gene expression during normal brain development as well as neurodegeneration. The electrophoretic mobility shift assay (EMSA) is a very powerful technique for studying changes of neuronal gene expression and determining protein: DNA interactions. EMSA can be used qualitatively to identify specific transcriptional or co-transcriptional factors in brain crude lysates or primary neurons and, in conjunction with mutagenesis, to identify the important binding sequences within a given gene. An advantage of studying protein: DNA interaction by an electrophoretic assay provides a better understanding of epigenetic changes during normal brain development and neurodegenerative process. [摘要]  转录或共转录因子与DNA的相互作用对于正常脑发育以及神经变性过程中神经元基因表达的变化是至关重要的。 电泳迁移率变动分析(EMSA)是一种非常强大的技术,用于研究神经元基因表达的变化和确定蛋白质:DNA相互作用。 EMSA可定性地用于鉴定脑粗提裂解物或原代神经元中的特定转录或共转录因子,并且与诱变相结合以鉴定给定基因内的重要结合序列。 通过电泳测定研究蛋白质:DNA相互作用的优点提供了对正常脑发育和神经变性过程中的表观遗传改变的更好理解。

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