| Polysome Preparation, RNA Isolation and Analysis
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Author:
Date:
2012-11-05
[Abstract] During mRNA translation, 40S and 60S ribosomal subunits bind to target mRNA forming into an 80S complex (monosome). This ribosome moves along the mRNA during translational elongation to facilitate tRNA reading codon, where translation is activated and many monosomes can bind the same mRNA simutaneously, which forms polysomes. Polysomes can be size-fractionated by sucrose density gradient centrifugation. The more specific mRNA in polysomes implies more active translational status of the mRNA.
[摘要] 在mRNA翻译期间，40S和60S核糖体亚基结合靶mRNA形成80S复合物（单体）。 这种核糖体在翻译延长过程中沿着mRNA移动以促进tRNA阅读密码子，其中翻译被激活并且许多单体可以同时结合相同的mRNA，其形成多核糖体。 多聚体可以通过蔗糖密度梯度离心进行大小分级。 多核糖体中更特异的mRNA意味着mRNA的更有活性的翻译状态。
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| In vitro RNA-protein Binding Assay by UV Crosslinking
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Author:
Date:
2012-11-05
[Abstract] Because covalent bond can form between RNA and its binding proteins after UV irradiation, UV cross-linking is widely used to identify the specific RNA binding proteins. This protocol is described in details as follows.
[摘要] 因为紫外线照射后可以在RNA及其结合蛋白之间形成共价键，所以紫外交联被广泛用于鉴定特异性RNA结合蛋白。 该协议详细描述如下。
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