Fetal bovine serum

胎牛血清

Company: Thermo Fisher Scientific

Catalog#: 11091-148

Immunohistochemistry Staining for Tumor-associated Macrophage Polarization in Murine Subcutaneous Colon Tumor Allografts

Gage J. Greening, Shelby N. Bess and Timothy J. Muldoon

Published online: 2018-12-05

Bio-protocol()

Company-protocol()

Other protocol()

Lung Clearance Assay

Author:

Pinchas Tsukerman and Ofer Mandelboim,

Date:

2013-03-05

[Abstract] Lung clearance assay tests the ability of innate immune cells (mainly NK cells) to eradicate in vivo cells injected via the tail vein of the mice. This assay helps to elucidate the role played by NK cells and their receptors (if the mice are genetically modified) against various human and mouse targets in an in vivo setting (Stern-Ginossar et al., 2008; Halfteck et al., 2009; Tsukerman et al., 2012).
[摘要] 肺清除测定测试先天免疫细胞（主要是NK细胞）根除通过小鼠尾静脉注射的体内细胞的能力。该测定有助于阐明NK细胞及其受体（如果小鼠被遗传修饰）在体内的各种人和小鼠靶标上发挥的作用（Stern-Ginossar等人，，2008; Halfteck等人，2009; Tsukerman等人，2012）。

Detection of CLEC5A-JEV Interaction by ELISA

Author:

Szu-Ting Chen and Shie-Liang Hsieh,

Date:

2012-10-05

[Abstract] JEV (Japanese encephalitis virus) belonging to Flaiviridae interacts with CLEC5A (C-type lectin domain family 5, member A), a member of C-type lectin associated with DAP12 signaling protein and expressed on myeloid cell, as the same extent as Dengue virus. This protocol is used to perform and determine the interaction between purified JEV particles with human and murine CLEC5A.Fc fusion proteins by ELISA method. The different strain and batch of purified JEV as well as purity of CLEC5A.Fc fusion protein might affect the result of absorbance. Modifications are most likely needed if users intend to perform ELISA assay. [摘要] 属于黄病毒科的JEV（病毒）与CLEC5A（C型凝集素结构域家族5，成员A） C型凝集素，与DAP12信号传导蛋白相关并且在骨髓细胞上表达，与登革热病毒的程度相同。该方案用于通过ELISA方法进行和确定纯化的JEV颗粒与人和鼠CLEC5A.Fc融合蛋白之间的相互作用。不同的菌株和批次的纯化JEV以及CLEC5A.Fc融合蛋白的纯度可能影响吸光度的结果。如果用户打算进行ELISA测定，则很可能需要修饰。

Comments

Fetal bovine serum

胎牛血清

免责声明