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DBCO-S-S-PEG3-Biotin conjugate

DBCO-S-S-PEG3-生物素缀合物

Company: Click Chemistry Tools
Catalog#: A112P3
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5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
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2012-08-05
[Abstract]  5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could in turn be used for high-throughput mapping via next-generation sequencing. [摘要]  5-羟甲基胞嘧啶(5-hmC)是哺乳动物基因组中新发现的DNA修饰。 这个协议是一种高效和选择性的化学方法来标记和捕获5-hmC,利用噬菌体酶,特别是添加葡萄糖部分到5-hmC,这可以反过来用于高通量映射通过下一个 - 生成测序。

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