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Author:
Date:
2016-06-05
[Abstract] This protocol is to differentially and simultaneously visualize both cells and airspaces in intact leaves and to create 3D structures of cells and airspaces from confocal images using the software DSLT_Demo (https://github.com/nslab2000/DSLT). Leaves stained with Nile Red in silicone-oil solution provide red color to cell membranes and green color to airspaces filled with silicone oil solution. This method is applicable to any tissues (except for dry seeds) of various plants including Arabidopsis, Nicotiana, Lemna and moss, and applicable even to hard leaves of plants such as switchgrass and Cinnamomum. Repeated use of this method enables time-lapse imaging of leaves over days and weeks ...
[摘要] 此协议是差异和同时可视化完整叶片的细胞和空间，并使用软件DSLT_Demo（ https://github.com/nslab2000/DSLT ）。 用尼罗红在硅油溶液中染色的叶子为细胞膜提供红色，并向填充有硅油溶液的空间提供绿色。 该方法适用于包括拟南芥 ，，， 和moss在内的各种植物的任何组织 硬叶植物如柳枝稷和樟脑。 重复使用这种方法使得叶片在几天和几周时间推移成像，因为尼罗红和硅油都对植物组织无害。
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