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Author:
Date:
2016-02-05
[Abstract] Cortico-cortical interactions play crucial roles in various brain functions. Here, we present a detailed surgical procedure for cortical voltage-sensitive dye (VSD) imaging that allows monitoring of spatiotemporal dynamics in cortical activity in living mice. Cortical neurons in the upper layers (layer 1-3) are stained with a VSD, and an image sensor with a fast sampling rate (500 Hz) detects fluorescent changes in corrective activity. The procedure includes fixing a mouse brain to a stereotaxic apparatus, craniotomy on a large cortical area, VSD staining, and wide-field imaging of cortical activity. The entire procedure can be completed in 5 h (from the administration of anesthesia to the start of cortical VSD imaging).
[摘要] 皮层皮层相互作用在各种大脑功能中起关键作用。 在这里，我们提供皮质电压敏感染料（VSD）成像允许监测活体小鼠皮质活动的时空动力学的详细的外科手术。 上层（层1-3）中的皮层神经元用VSD染色，并且具有快速采样率（500Hz）的图像传感器检测校正活性中的荧光变化。 该程序包括将小鼠脑固定到立体定位装置，在大皮质区域上的颅骨切开术，VSD染色和皮层活动的宽视野成像。 整个过程可以在5小时内完成（从麻醉的施用到皮质VSD成像的开始）。
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