|
Author:
Date:
2015-11-20
[Abstract] The inflammatory response is essential to the reestablishment of cutaneous homeostasis following injury. In this context, leukocytes arrive at the wound site and orchestrate essential events in the wound healing process. Therefore, the quantification of specific subsets of inflammatory cells in the wound tissue is of considerable interest. The current protocol focus on a quantitative index of neutrophils and macrophages accumulation within skin lesions by measuring the specific activity of the marker enzymes Myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAG), respectively. MPO is present in high levels in the azurophilic granules of neutrophils and NAG in lysosomes of activated macrophages. These methods allow the indirect estimation of the abundance of neutrophils and ...
[摘要] 炎症反应对于损伤后皮肤稳态的再建立是必需的。 在这种情况下，白细胞到达伤口部位并协调伤口愈合过程中的重要事件。 因此，对伤口组织中炎性细胞的特定亚群的定量是相当感兴趣的。 通过测量标记酶髓过氧化物酶（MPO）和N-乙酰基-β-D-氨基葡萄糖苷酶（NAG）的比活性，目前的方案集中于皮肤损伤中嗜中性粒细胞和巨噬细胞积累的定量指数。 MPO以高水平存在于嗜中性粒细胞和NAG的嗜苯胺粒细胞中，在激活的巨噬细胞的溶酶体中。 这些方法允许间接估计积累到皮肤中的嗜中性粒细胞和巨噬细胞的丰度。
|