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10% Ketamine Hydrochloride

10%盐酸氯胺酮

Company: Agropecuária Tarumã
Catalog#: 8565
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Skin Wound Healing Model - Excisional Wounding and Assessment of Lesion Area
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Date:
2015-11-20
[Abstract]  This protocol focus on the most common surgical mouse model of cutaneous excisional wound healing used to study the cellular and molecular pathways involved in wound repair and regeneration as well as in translational applications such as the evaluation of new therapeutic modalities. This model allows the monitoring of the wound closure and the tissue collection for histological and molecular analyses. Briefly, full skin thickness excisional wounds are created on the dorsum of the mouse as the excision extends through the panniculosus carnosus. Wounds larger and minor diameters are then regularly measured and wound closure rate is calculated based on wound area relative to the original size. [摘要]  该协议侧重于最常见的皮肤切除伤口愈合的外科小鼠模型,用于研究涉及伤口修复和再生以及翻译应用(例如评价新的治疗方式)的细胞和分子途径。 该模型允许监测伤口闭合和组织收集用于组织学和分子分析。 简而言之,当小鼠的背部延伸穿过肉芽肿时,在小鼠的背部产生完全皮肤厚度的切除伤口。 然后定期测量伤口的较大和较小直径,并基于相对于原始尺寸的伤口面积计算伤口闭合率。

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