Isolation and Culture of Human Adipose-derived Stem Cells from Subcutaneous and Visceral White Adipose Tissue Compartments
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Author:
Date:
2016-11-20
[Abstract] Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human white adipose tissue (WAT) has great advantages due to its rich tissue source and simple surgical procedure. In this detailed protocol we describe a protocol to isolate and characterize ASCs from human WAT. Molecular characterization of isolated ASCs was performed through surface marker expression profiling using flow cytometry. Adipogenic capacity of the isolated ASCs was confirmed through inducing adipogenic differentiation and Oil Red O ...
[摘要] Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human white adipose tissue (WAT) has great advantages due to its rich tissue source and simple surgical procedure. In this detailed protocol we describe a protocol to isolate and characterize ASCs from human WAT. Molecular characterization of isolated ASCs ...
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Quantifying the Permeability of the Apoplastic Water Barrier in Cosmos Petals
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Author:
Date:
2015-11-20
[Abstract] The capacity of plants to minimize uncontrolled water loss is essential for survival in adverse and changing climatic conditions. In order to assess and compare the effectiveness of apoplastic barriers to water, the permeability of the barrier must first be quantified. Studies have accomplished this directly by quantifying tritium flux or indirectly by measuring the influx/efflux of water surrogates such as dyes, chlorophyll, and herbicides. Other studies have relied on comparative methods such as survival rates after drought. These methods rely on radioactive material, correlations, or qualitative comparisons. However, a quantitative method is necessary that directly measures water efflux and that allows easy comparisons within and between experiments, plant parts, plant species, and ...
[摘要] 植物使不受控制的水损失最小化的能力对于在不利和变化的气候条件下的存活是必要的。为了评估和比较非水性阻隔层对水的有效性,必须首先量化阻隔层的渗透性。研究直接通过量化氚通量或通过测量水替代物如染料,叶绿素和除草剂的流入/流出间接实现了这一点。其他研究依赖于比较方法,如干旱后的存活率。这些方法依赖于放射性材料,相关性或定性比较。然而,定量方法是必要的,直接测量水流出,并允许在实验,植物部分,植物物种,特别是研究实验室内和之间的简单比较。这里我们详细介绍了Schönherr和Lendzian(1981)首次描述的重量分析方法,它可以在不到半天内完成,并且根据植物屏障在一到十天内完成。这种方法已经用于许多关于叶和果皮表皮的研究中,并且最近也用于来自宇宙的花瓣( Cosmos bipinnatus ; Buschhaus ,2015)。
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