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Ascorbate Oxidase from Cucurbita sp.

南瓜属的抗坏血酸氧化酶

Company: Sigma-Aldrich
Catalog#: A0157
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Detection and Measurement of ROS in Tobacco Leaves
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2013-03-05
[Abstract]  Leaf metabolism produces hydrogen peroxide (H2O2) at high rates, high level H2O2 accumulation can cause oxidative stress. This protocol describes a method for determining H2O2 concentration in tobacco leaves. In this method all extractions were performed with HClO4, neutralized, and pretreated with ascorbate oxidase to eliminate ascorbate interferences. H2O2 content was determined using a colorimetric assay spiked with an internal control. Interfering peroxides were determined in parallel using a negative control treated with catalase and subsequently subtracted. [摘要]  叶代谢以高速率产生过氧化氢(H 2 O 2 O 2),高水平的H 2 O 2 O 2积累 可引起氧化应激。 该方案描述了用于测定烟草叶中H 2 O 2 O 2浓度的方法。 在该方法中,所有提取用HClO 4进行,中和,并用抗坏血酸氧化酶预处理以消除抗坏血酸盐干扰。 使用掺入内部对照的比色测定法测定H 2 O 2含量。 使用用过氧化氢酶处理的阴性对照平行测定干扰过氧化物,随后扣除。

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