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Author:
Date:
2015-10-05
[Abstract] Silicon (Si) is a biologically important element for plants in the order Poales (Yamamoto et al., 2011; Kido et al., 2015). In rice, Si is mainly deposited in the motor cells and the cell walls of the leaf epidermis. However, the molecular basis of this overall process has not been elucidated. Thus, we propose a protocol for the histochemical staining of the silica body based on specific hydrogen bonding between silanol group and the carboxylate group of crystal violet lactone (Ichimura et al., 2008), as described by Isa et al. (2010), but with minor modifications. This modified protocol can be used for observing Si accumulation during rice development.
[摘要] 硅（Si）对于Poales（Yamamoto等人，2011; Kido等人，2015年）的植物是重要的生物元素。 在稻中，Si主要沉积在运动细胞和叶表皮的细胞壁中。 然而，该整个方法的分子基础尚未阐明。 因此，我们提出了用于基于硅烷醇基团和结晶紫内酯的羧酸酯基团之间的特异性氢键的二氧化硅体的组织化学染色的方案（Ichimura等人，2008），如Isa （2010），但稍作修改。 这个修改的协议可以用于观察水稻开发过程中的硅积累。
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