| Experimental Design to Determine Drought Stress Response and Early Leaf Senescence in Barley (Hordeum vulgare L.)
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Author:
Date:
2016-03-05
[Abstract] Premature leaf senescence induced by drought stress is a main factor for yield losses in barley. Research in drought stress tolerance has become more important as due to climate change the number of drought periods will increase and tolerance to drought stress has become a goal of high interest in barley breeding. However, reliable screening for drought stress tolerance is still a difficult task. This protocol describes the experimental design for the phenotyping for drought stress tolerance and early leaf senescence in the juvenile stage of barley (A) and the determination of six physiological parameters involved in drought tolerance and leaf senescence (B to G) according to Wehner et al., (2015).
[摘要] 由干旱胁迫诱导的过早叶子衰老是大麦产量损失的主要因素。 干旱胁迫耐受性的研究变得更加重要，因为由于气候变化，干旱期的数量将增加，并且对干旱胁迫的耐受性已经成为大麦育种中高度关注的目标。 然而，对干旱胁迫耐受性的可靠筛选仍然是一项艰巨的任务。 该协议描述了针对大麦幼龄阶段（A）的干旱胁迫耐受性和早期叶衰老的表型的实验设计以及根据Wehner等人（2006）的参考干旱耐受性和叶衰老（B对G）的六个生理参数的确定 > et al。，（2015）。
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| 5’ Rapid Amplification of cDNA Ends (5’ RACE) of Agrobacterial T-DNA Genes within Transformed Plant Sample
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Author:
Date:
2015-09-20
[Abstract] The T-DNA (transferred-DNA) region of virulent Agrobacterium tumefaciens (A. tumefaciens) strain is transferred and integrated into the plant genome, and thereby the T-DNA genes are expressed in transformed plant cells. This protocol was used to analyze the transcription start sites (TSSs) of agrobacterial T-DNA genes within plant crown gall tumor. Firstly, the stems of Arabidopsis thaliana were inoculated by A. tumefaciens strain C58 and developed crown gall tumor. Subsequently, the mRNA was extracted from the crown gall tumor and then used for amplification of 5’ cDNA ends by 5’ Rapid Amplification of cDNA Ends (5’ RACE) assay. The full-length cDNAs were generated in reverse transcription reactions and used to analyze TSSs. Here, TSSs of three ...
[摘要] 将有毒的根癌土壤杆菌（根瘤土壤杆菌）菌株的T-DNA（转移的DNA）区转移并整合到植物基因组中，从而T-DNA基因在转化的植物细胞中表达。该方案用于分析植物冠gall肿瘤内农杆菌T-DNA基因的转录起始位点（TSS）。首先，通过接种拟南芥的茎。 tumefaciens 菌株C58和发展的冠gall肿瘤。随后，从冠gall瘤提取mRNA，然后通过cDNA末端的快速扩增（5'RACE）测定用于扩增5'cDNA末端。全长cDNA在逆转录反应中产生并用于分析TSS。这里，作为实例分析了三种癌基因的TSS，IaaH ，IaaM 和 Ipt 。该协议还允许鉴定在植物细胞中表达的其他农杆菌T-DNA基因的TSS
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