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Author:
Date:
2015-09-20
[Abstract] Visualization of nuclei in S-phase cells in tissues is important for not only cell cycle research but also developmental research because morphogenesis is usually achieved by a combination of cell proliferation and cell expansion. Recently, DNA labeling with 5-ethynyl-2′-deoxyuridine (EdU), which is an analog of thymidine, has been used to visualize nuclei in S-phase cells to assess the activity of cell proliferation during development of plants. EdU is efficiently incorporated into newly synthesized DNA, and detection of EdU is based on the covalent reaction between EdU and Alexa Fluor® dye, which is one of useful fluorescent dyes; this allows us to use mild conditions for the assay without any DNA denaturation. This method could be easily applicable, and, indeed, has been ...
[摘要] 组织中S期细胞中核的可视化对于不仅细胞周期研究而且对发育研究是重要的，因为形态发生通常通过细胞增殖和细胞扩增的组合来实现。 最近，用5-乙炔基-2'-脱氧尿苷（EdU）（其是胸苷的类似物）的DNA标记已用于在S期细胞中显现细胞核以评估植物发育过程中细胞增殖的活性。 EdU有效地并入新合成的DNA中，并且EdU的检测基于EdU和作为有用的荧光染料之一的Alexa Fluor 染料之间的共价反应; 这允许我们使用温和条件进行测定而没有任何DNA变性。 该方法可以容易地应用，并且实际上已经用于各种模型和非模型植物物种。 在这里，我们已经描述了一个协议开发用于检测叶中S期细胞中的核。
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