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Pertussis toxin(PTX)

百日咳毒素(PTX)

Company: List Biological Labs
Catalog#: 183
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Calcium Mobilisation Assay in Response to Chemokine Stimulation
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2012-05-05
[Abstract]  This assay is used to measure calcium mobilization in lymphocytes (either in primary cells or cell lines) in response to chemokine stimulation using ratiometric analysis. It has also been used for measuring TCR mediated calcium flux. In addition, the same labeling procedure with the addition of brilliant black (a quenching agent) (Sigma-Aldrich, catalog number: 211842) in the loading buffer (at 100 μM) allows for quantification using the FLIPR system on poly-D-lysine plates. Probenicid is an anion-exchange protein inhibitor and prevents the extrusion of the dyes by organic ion transporters. [摘要]  该测定用于使用比率分析测量响应趋化因子刺激的淋巴细胞(原代细胞或细胞系)中的钙动员。 它也被用于测量TCR介导的钙通量。 此外,在加样缓冲液(100μM)中加入亮黑(淬灭剂)(Sigma-Aldrich,目录号:211842)的相同标记方法允许使用FLIPR系统定量聚D-赖氨酸 板。 丙磺舒是阴离子交换蛋白抑制剂,并通过有机离子转运蛋白防止染料的挤出。

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