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Author:
Date:
2015-09-05
[Abstract] Plant protein expression can be a challenging enterprise in any biochemical or molecular biology research project. Several heterologous systems like bacteria, yeast, insect cells and cell free systems have been used to produce plant proteins for in vitro experiments and structural characterization. However, due to particularities of plant proteins, for example the specific type and abundance of post-translational modifications (e.g. glycosylation), a plant system to express plant proteins is extremely desirable. The use of Nicotiana benthamiana (N. benthamiana) plants for protein expression has proven to be quick and reliable. To illustrate the robustness and rapidity of this system, recent efforts to produce the first protein based drug against the ...
[摘要] 植物蛋白表达可以是任何生物化学或分子生物学研究项目中的挑战性企业。几种异源系统如细菌，酵母，昆虫细胞和无细胞系统已被用于生产植物蛋白用于体外实验和结构表征。然而，由于植物蛋白的特殊性，例如翻译后修饰（例如糖基化）的特异性类型和丰度，表达植物蛋白的植物系统是非常需要的。已经证明使用本氏烟草（本生烟草）植物进行蛋白质表达是快速和可靠的。为了说明该系统的稳健性和快速性，最近在N中进行了生产针对埃博拉病毒的第一种基于蛋白质的药物的努力。本草案蛋白质表达系统（Choi等人，2015）。
该方案描述了一种简单的系统，用于表达和富集（亲和纯化） > N。本生烟草叶，其被成功地用于拟南芥果胶乙酰酯酶，PAE8和PAE9的表征中（de Souza等人，2014）。
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